Biosynthesis of Di- myo -Inositol-1,1′-Phosphate, a Novel Osmolyte in Hyperthermophilic Archaea
نویسندگان
چکیده
منابع مشابه
Restoration of the di-myo-inositol-phosphate pathway in the piezo-hyperthermophilic archaeon Thermococcus barophilus.
Most Thermococcales accumulate di-myo-inositol-phosphate (DIP) as an organic solute as a response to heat stress. We have studied the accumulation of this osmolyte in the high-hydrostatic pressure adapted hyperthermophile Thermococcus barophilus. We found no accumulation of DIP under any of the stress conditions tested, although this archaeon harbors the 3 DIP synthesis genes. Lack of synthesis...
متن کاملEvolution of the biosynthesis of di-myo-inositol phosphate, a marker of adaptation to hot marine environmentsemi_2621 1..11
Luís G. Gonçalves,1 Nuno Borges,1 François Serra,2 Pedro L. Fernandes,3 Hernán Dopazo2* and Helena Santos1* Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, Apartado 127, 2780-157 Oeiras, Portugal. Evolutionary Genomics Laboratory, Bioinformatics & Genomics Department, Centro de Investigación Príncipe Felipe, Valencia 46012, Spain. Instituto Gulben...
متن کاملGenomic identification and in vitro reconstitution of a complete biosynthetic pathway for the osmolyte di-myo-inositol-phosphate.
Di-myo-inositol 1,1'-phosphate (DIP) is a major osmoprotecting metabolite in a number of hyperthermophilic species of archaea and bacteria. Although the DIP biosynthesis pathway was previously proposed, genes encoding only two of the four required enzymes, inositol-1-phosphate synthase and inositol monophosphatase, were identified. In this study we used a comparative genomic analysis to predict...
متن کاملNew compatible solutes related to Di-myo-inositol-phosphate in members of the order Thermotogales.
The accumulation of intracellular organic solutes was examined in six species of the order Thermotogales by nuclear magnetic resonance spectroscopy. The newly discovered compounds di-2-O-beta-mannosyl-di-myo-inositol-1,1'(3,3')-phosphate and di-myo-inositol-1,3'-phosphate were identified in Thermotoga maritima and Thermotoga neapolitana. In the latter species, at the optimum temperature and sal...
متن کاملEnantiomeric Form of myo-Inositol-1-Phosphate Produced by myo-Inositol-1-Phosphate Synthase and myo-Inositol Kinase in Higher Plants.
The product of myo-inositol-1-phosphate synthase, EC 5.5.1.4, from mature pollen of Lilium longiflorum Thunb., cv Ace (Easter lily) and that of myo-inositol kinase, EC 2.7.1.64, from wheat germ has been identified as 1l-myo-inositol-1-phosphate by gas chromatography of its trimethylsilyl-methyl phosphate derivative on a glass capillary column bearing a chiral phase.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1998
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.180.15.3785-3792.1998